Acute myocardial infarction is one of the important reasons of death and unhealthiness in the world. Acute Myocardial infarction signifies sudden necrosis or death of a portion of cardiac muscle due to inadequate blood supply. The present study was undertaken to investigate the changes in serum lipids in patients with acute myocardial infarction in the Department of Biochemistry, Government General Hospital, Rajiv Gandhi Institute of Medical Sciences, Adilabad. The levels of lipid profile were significantly changed in the acute myocardial infarction patients. Acute myocardial infarction patients had significantly higher levels of total cholesterol, LDL-cholesterol, TG, and lower level of HDL-cholesterol, as compared to the control subjects. We found a significant association of lipid profiles with acute myocardial infarction. Reduced serum HDL-cholesterol and increased Serum LDL, Serum TG, in our study subjects may be the effective reasonable lipid disorders in AMI patients.