Babesiosis caused by B. gibsoni and Babesia canis is a tick borne protozoal parasite prevalent in the canines worldwide. In the current study, a total of 432 blood samples, were collected and examined at Veterinary Polyclinic, Jalandhar (Punjab) during April to December. Examination of Giemsa stained peripheral blood smears revealed that 6.01% (26/432) of canines were positive for canine babesiosis comprising of B. gibsoni (5.78%) and B. canis infections (0.23%).