The effect of VAM fungi on Calcium uptake in three cultivars of Okra was recorded by giving inoculation treatment of Glomus fasciculatum and Gigaspora gigantea. The data revealed that in cultivar Parbhani kranti, non-mycorrhizal plant has shown less amount of calcium uptake in mg per 100 gm, while the plants inoculated by Glomus fasciculatum and Gigaspora gigantea shown maximum uptake. In Arkanamika and Selection-51also the high amount of calcium was noted in mycorrhizal plants than in non mycorrhizal plant.