Background: Chronic suppurative otitis media (CSOM) is a potentially serious disease because of its complications. Change in the bacteriological profile with indiscriminate use of antimicrobial agents has been associated with the emergence of multiple drug resistant strains. Objective: The aim of the study is to isolate & identify the common bacteria causing CSOM & determine their antimicrobial susceptibility pattern. Materials & Methods: Ear swabs were collected & cultured from 55 patients with purulent discharge from perforated tympanic membranes attending ENT OPD. The organisms isolated were identified by standard microbiological methods & antibiogram pattern was determined. Results: Analysis of bacterial flora of the present study showed predominance of Gram negative bacilli (65.45%). The most common organism isolated was Pseudomonas aeruginosa (40%) followed by Staphylococcus aureus (20%). Five samples grew anaerobes (9.09%). Ceftazidime was found to be the most effective drug followed by amikacin. All the anaerobic bacteria were susceptible to metronidazole. Conclusion: The knowledge of the local pattern of the infection is essential for starting empirical therapy and reducing the potential risks of complications.