This study aims to determine whether additional of lactic acid bacteria from sewage force feeding fish can lower blood levels of HDL broiler. Microbes are a source of research material LAB isolates isolated from fish waste faeces. And chicken used was strain Lohmann male broiler production PT. Multi Breeder Adirama as many as 40 fish age 1 day. which is divided into 4 treatments with each treatment consisted of 10 individuals as replicates, were taken randomly and maintained for 42 days.Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 10 7CFU / ml. , IV treatment BAL cell count was 10 8 CFU is / ml. RESULTS: Treatment administration of lactic acid bacteria are not significantly different (P <0.05) on HDL blood of broiler chickens. So the administration of lactic acid bacteria does not affect blood levels of HDL broiler.