Introduction: Urinary Tract Infection (UTI) is the most common bacterial infection in human population and also one of the most frequently occurring nosocomial infection. The prevalence of UTI depends on age, sex, comorbid conditions, genital hygiene etc. Aims: Present study was conducted to determine the spectrum of causative agents responsible for UTI and to detect the extent of drug resistance. Methodology: The present cross sectional study was conducted in Government Medical College and Hospital, Akola (Maharashtra, India). The data was collected from 1st January 2013 to 31st December 2014. A total of 261 clean catch, mid-stream urine (10 ml) samples were collected according to study protocol. Results: In the present study, urine samples from a total of 261 UTI patients were taken for study. Out of them 119 (45.6%) samples belonged to male and 142 (54.4%) belonged to female patients. Out of 261 urine samples, 132 (50.6%) showed significant bacteriuria. Among the tested antibiotics the highest susceptibility shown by the Gram negative bacteria was forPiperacillin-tazobactam, Imipenem, Amikacin and Gentamicin. Amongst Gram positive bacteria’s Staphylococcus aureus was commonest isolate showing highest susceptibility to Linezolid and Teicoplanin (81.8%). Conclusion: As drug resistance among bacterial pathogens is an evolving process, regular surveillance and monitoring is necessary to provide physician’s knowledge on the updated and most effective empirical treatment of UTIs.
