Sensitivity of isolated pathogenic bacteria for aqueous and alcohl extract of cassia senna

In view of the fact that ancient time, plants have been a tremendous source of medicine. The knowledge of traditional medicine and medicinal plants and their study of scientific chemical principles may lead to the discovery of newer and cheaper drugs.Cassia was observed to have antibacterial activity and can be used to combat against vast flora of microorganisms. Senna (Cassia) has wide range of pharmacological actions hence present study was undertaken to evaluate the antibacterial potential of methanolic and aqueous extracts of dry flowers of Cassia using agar well, and disc diffusion methods. The microorganisms used include Staphylococcus aureus; E. coli; Pseudomonas aeruginosa; Bacillus subtilius; Klebsiella and Proteus. All extract established significant antibacterial activity against tested bacteria. Size of growth inhibition zone showed significant difference. Of present study revealed that in aqueous extract well method, Proteus showed the highest sensitivity, followed by each of E. coli, Pseudomonas aeruginosa, and Klebsiella, while Bacillus and Staphy. showed no significant difference .Aqueous disc method Pseudomonas aeruginosa and Proteus the most sensitive bacteria, other E. Coli, Bacillus, Klebsiella, Staphylococcus aeureus no significant changes. Aqueous extract in comparison between well and disc methods only Bacillus at 50 and 100 mg / ml, and Proteus art 100 was significantly different.Alcohol extract well method: E. coli was the most sensitive, followed by each of Bacillus, Staphylococcus, Pseudomonas, Klebsiella and Proteus which were sensitive at 100 mg / ml in comparison with 50 mg / ml. Disc method: Proteus was the most sensitive followedby Pseudomonas, then each of E. coli and Klebsiella. While Staphylococcus and Bacillus showed no significant difference. Sensitivity to alcohol extract in comparison between well and disc methods E. coli, Staphylococcus and Proteus were the most sensitive. While Pseudomonas was significantly sensitive only at 25 mg / ml. Bacillus and Klebsiella not show significant difference. In comparison between aqueous and alcohol extract the results revealed that in well method, Bacillus; staphylococcus; E. Coli and Klebsiella showed no significant differences; Proteus significant at the 25, 50 and 100 mg / ml. Pseudomonas showed significantly at 100 mg / ml .While in disc method all the test bacteria showed no significant differences.

Author: 
Al-Khafaji Nazar Jabbar, Al-Zubaedi Raad Mahmood and Al-Khafaji Mayada Nazar
Journal Name: 
Int J Inf Res Rev
Volume No: 
02
Issue No: 
09
Year: 
2015
Paper Number: 
0501
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