The present study was undertaken to evaluate the preliminary phytochemical constitution and invitro-antimicrobial activity of Aeglemarmelos (L.) Correa leaf extract. Preliminary phytochemical screening of the freshly prepared plant leaf extract showed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids and steroids. The invitro-antimicrobial activity of the successive leaf extracts of Aeglemarmelos (L.) Correa was studied in petroleum ether, chloroform, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive and gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method and Agar disc diffusion method were used to evaluate the antibacterial efficacy. The Minimum inhibitory concentration (MIC) of all these solvent extracts of said plant was determined by Agar well diffusion method. The reference antibiotics Chloramphenicol and Ampicillin (Antibacterial); Nystatinand Clotrimazole (Antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts. The invitro-antimicrobial activity study showed that all the seven successive extracts of the leaf powder of Aeglemarmelos (L.) Correa, exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. Highly polar solvents ie.ethanol, methanol and water showed the most significant antibacterial and antifungal activity against all tested organisms. The results of the study revealed that the leaf powder of Aeglemarmelos (L.) Correa can be considered as a possible source of various phytochemical constituents having an in-vitro antimicrobial potential.