The experiment was conducted at ASPEE, Agricultural Research and Development Foundation, Village Nare, Taluka Wada, District Palghar, Maharashtra, India. In this study, the effects of different levels of sucrose in vase solution treatments on post harvest solution uptake, florets diameter, vase life of spike of gladiolus cv. American Beauty were carried out. The 5.5 % vase solution treatment of sucrose resulted in maximum vase solution uptake (57, 56 and 56.50ml), florets diameter (6.22, 6.54 and 6.38cm), number of opened florets percentage (62.17, 65 and 63.58%), vase life of spike (12.04, 12.44 and 12.24 days) and minimum percentage of unopened florets per spike (37.83, 35 and 36.42%), respectively in the both trials under laboratory condition and also pooled study of gladiolus cv. American Beauty and then followed by treatment T10 .