Anemia is present in the majority of patients with chronic renal failure (CRF) on hemodialysis, The proximate cause of the anemia is an inadequate endogenous erythropoietin (EPO), iron is essential for hemoglobin formation and productive erythropoiesis, accurately assessing iron status is a prerequisite for diagnosing iron deficiency, monitoring the response to iron supplementation, and maintaining effective erythropoiesis in these patients. Objectives: The aim of this study is to assess iron profile in patient with CRF undergoing hemodialysis and to correlate iron profile result with patient’s age, gender and duration of dialysis. Materials and Methods: A descriptive analytical case control study was performed in Ibn Sina Hospital, Khartoum, Sudan. A total of 80 Sudanese were enrolled in this study (40 CRF patients under hemodialysis and 40 healthy controls), 51 (64%) were male and 29 (36%) were females, their age ranged between 20 to 85 years. Serum iron, saturated transferrin and total iron binding capacity (TIBC) were measured using Biosystem 350 semi-automated spectrophotometer and serum ferritin was measured using Roche Elecsys 2010. Data were analyzed by using statistical package forsocial sciences (SPSS) verssion16. Results: The present study showed that serum iron and serumferritin of those under hemodialysis patient were significantlyhigher compared to control group (P value 0.00) for both.Also TIBC and transferrin saturated were significantly lowered compared to control group (p value 0.00) for both Conclusion: Patients with CRF andunder hemodialysis are prone to iron overload.